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primescripttm rt master mix kit  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm rt master mix kit
    Primescripttm Rt Master Mix Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 26640 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+rt+master+mix+kit/PrimeScript+RT+Master+Mix/pmc13035274-172-7-15
    Average 99 stars, based on 26640 article reviews
    primescripttm rt master mix kit - by Bioz Stars, 2026-09
    99/100 stars

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    Expressing:

    Article Title: Andrographis restrains the progression of colorectal cancer by inhibiting the KLF3/LEMD1 axis.
    Article Snippet: .. LEMD1 and KLF3 mRNA expression was determined using RT-qPCR. cDNA was prepared using the PrimeScript RT Master Mix kit (RR036A, Takara, Japan). .. LEMD1 mRNA level was detected with the SYBR Premix Ex Taq II (RR820A, Takara) on the Thermal Cycler DiceTM Real Time System (Takara).

    Agarose Gel Electrophoresis:

    Article Title: Modulatory effects of tea-derived bioactives on oral microbiota and their virulence-reducing properties.
    Article Snippet: RNA was extracted using an Eastep Super Total RNA Extraction Kit (Promega USA) according to the manufacturer’s instructions. .. RNA concentrations and purities were determined by UVspectrophotometry (Implen NanoPhotometer N50, Germany), and agarose gel electrophoresis was used to detect RNA integrity. cDNA synthesis was conducted using a PrimeScript RT Master Mix Kit (Takara Japan) in a 20 μl reaction mixture. .. Reverse transcription qPCR (RT-qPCR) was conducted on a MiniOption System (Bio-Rad, USA).

    cDNA Synthesis:

    Article Title: Modulatory effects of tea-derived bioactives on oral microbiota and their virulence-reducing properties.
    Article Snippet: RNA was extracted using an Eastep Super Total RNA Extraction Kit (Promega USA) according to the manufacturer’s instructions. .. RNA concentrations and purities were determined by UVspectrophotometry (Implen NanoPhotometer N50, Germany), and agarose gel electrophoresis was used to detect RNA integrity. cDNA synthesis was conducted using a PrimeScript RT Master Mix Kit (Takara Japan) in a 20 μl reaction mixture. .. Reverse transcription qPCR (RT-qPCR) was conducted on a MiniOption System (Bio-Rad, USA).

    Spectrophotometry:

    Article Title: Genome-wide analysis of the AP2/ERF gene family in Rheum officinale Baill.: Evolution and expression profiling during plant development, abiotic stresses, and exogenous hormone responses.
    Article Snippet: Total RNA was extracted from R. officinale seedlings using a Plant RNA Rapid Extraction Kit (Vazyme, China). .. RNA quality was assessed using a NanoDrop spectrophotometer and an Agilent 2100 Bioanalyzer. cDNA was synthesized using the PrimeScript RT Master Mix kit (TaKaRa). ..

    Synthesized:

    Article Title: Genome-wide analysis of the AP2/ERF gene family in Rheum officinale Baill.: Evolution and expression profiling during plant development, abiotic stresses, and exogenous hormone responses.
    Article Snippet: Total RNA was extracted from R. officinale seedlings using a Plant RNA Rapid Extraction Kit (Vazyme, China). .. RNA quality was assessed using a NanoDrop spectrophotometer and an Agilent 2100 Bioanalyzer. cDNA was synthesized using the PrimeScript RT Master Mix kit (TaKaRa). ..

    Article Title: Identifying candidate genes for grain protein content in wheat (Triticum aestivum L.) by integrated GWAS and transcriptome analysis.
    Article Snippet: .. Functional enrichment analyses, including GO and KEGG pathway enrichment, were performed to identify DEGs significantly enriched in GO terms and metabolic pathways using Goatools and KOBAS [49], respectively, with a Bonferroni-corrected P-value ≤ 0.05 as the significance threshold. qRT-PCR analysis cDNA was synthesized using the TaKaRa PrimeScript RT Master Mix Kit, and all reaction steps were carried out strictly in accordance with the manufacturer’s instructions. .. Primers were designed using NCBI (Table S10). qRT-PCR was performed using the TaKaRa TB Green Premix Ex Taq II Kit on a LightCycler 480 system (Roche), with Tubulin used as the reference gene.

    Article Title: Enhanced Auricular Cartilage Regeneration via 3D-Printed Hydrogel With miR-92a-3p-Enriched Platelet-Rich Plasma-Derived Extracellular Vesicles.
    Article Snippet: Auricular cartilage reconstruction in microtia remains a formidable challenge in regenerative medicine.. Although cartilage tissue engineering holds substantial promise, achieving reliable and efficient chondrogenesis remains a pressing challenge owing to the intrinsically quiescent phenotype of chondrocytes.. We present engineered platelet-derived extracellular vesicles (PEVs) that enhance auricular chondrocyte chondrogenesis in a 3D-printed hydrogel, enabling safe and efficient ear regeneration.

    Article Title: Txnip regulates glycolysis and tricarboxylic acid cycle balance to maintain bone homeostasis.
    Article Snippet: .. Following sufficient grinding and centrifugation at 12,000×g for 10 min, the supernatant was combined with isopropanol for 5 min, and the subsequent procedures were conducted using the Kit. cDNA was synthesized from the RNA template using the PrimeScript RT Master Mix kit (Takara, Cat # RR036A) for reverse transcription. .. Gene expression was analyzed using the TBGreen® Premix Ex TaqTM kit (Takara, Cat # RR420A).

    Article Title: Loss of astrocytic Bmal1 promotes blood-brain barrier disruption and synaptic dysfunction during systemic inflammation.
    Article Snippet: Total RNA was isolated from brain tissues using TRIzol reagent (Invitrogen, 15596018) according to the manufacturer’s instructions. .. Complementary DNA (cDNA) was synthesized from the extracted RNA using the PrimeScript RT Master Mix Kit (Takara, RR036A). .. Quantitative real-time PCR (qRT-PCR) was performed on a QuantStudio 3 Real-Time PCR System (Applied Biosystems, USA) using SYBR Green I Master Mix (ABclonal, RK21203).

    Functional Assay:

    Article Title: Identifying candidate genes for grain protein content in wheat (Triticum aestivum L.) by integrated GWAS and transcriptome analysis.
    Article Snippet: .. Functional enrichment analyses, including GO and KEGG pathway enrichment, were performed to identify DEGs significantly enriched in GO terms and metabolic pathways using Goatools and KOBAS [49], respectively, with a Bonferroni-corrected P-value ≤ 0.05 as the significance threshold. qRT-PCR analysis cDNA was synthesized using the TaKaRa PrimeScript RT Master Mix Kit, and all reaction steps were carried out strictly in accordance with the manufacturer’s instructions. .. Primers were designed using NCBI (Table S10). qRT-PCR was performed using the TaKaRa TB Green Premix Ex Taq II Kit on a LightCycler 480 system (Roche), with Tubulin used as the reference gene.

    Significance Assay:

    Article Title: Identifying candidate genes for grain protein content in wheat (Triticum aestivum L.) by integrated GWAS and transcriptome analysis.
    Article Snippet: .. Functional enrichment analyses, including GO and KEGG pathway enrichment, were performed to identify DEGs significantly enriched in GO terms and metabolic pathways using Goatools and KOBAS [49], respectively, with a Bonferroni-corrected P-value ≤ 0.05 as the significance threshold. qRT-PCR analysis cDNA was synthesized using the TaKaRa PrimeScript RT Master Mix Kit, and all reaction steps were carried out strictly in accordance with the manufacturer’s instructions. .. Primers were designed using NCBI (Table S10). qRT-PCR was performed using the TaKaRa TB Green Premix Ex Taq II Kit on a LightCycler 480 system (Roche), with Tubulin used as the reference gene.

    Quantitative RT-PCR:

    Article Title: Identifying candidate genes for grain protein content in wheat (Triticum aestivum L.) by integrated GWAS and transcriptome analysis.
    Article Snippet: .. Functional enrichment analyses, including GO and KEGG pathway enrichment, were performed to identify DEGs significantly enriched in GO terms and metabolic pathways using Goatools and KOBAS [49], respectively, with a Bonferroni-corrected P-value ≤ 0.05 as the significance threshold. qRT-PCR analysis cDNA was synthesized using the TaKaRa PrimeScript RT Master Mix Kit, and all reaction steps were carried out strictly in accordance with the manufacturer’s instructions. .. Primers were designed using NCBI (Table S10). qRT-PCR was performed using the TaKaRa TB Green Premix Ex Taq II Kit on a LightCycler 480 system (Roche), with Tubulin used as the reference gene.

    Purification:

    Article Title: Enhanced Auricular Cartilage Regeneration via 3D-Printed Hydrogel With miR-92a-3p-Enriched Platelet-Rich Plasma-Derived Extracellular Vesicles.
    Article Snippet: Auricular cartilage reconstruction in microtia remains a formidable challenge in regenerative medicine.. Although cartilage tissue engineering holds substantial promise, achieving reliable and efficient chondrogenesis remains a pressing challenge owing to the intrinsically quiescent phenotype of chondrocytes.. We present engineered platelet-derived extracellular vesicles (PEVs) that enhance auricular chondrocyte chondrogenesis in a 3D-printed hydrogel, enabling safe and efficient ear regeneration.

    Article Title: Deciphering IFN signaling in gastric cancer: A single-cell and bulk transcriptomic integration reveals CXCR4 as a key immunomodulator and prognostic determinant
    Article Snippet: Total RNA extraction was performed with Trizol reagent (Invitrogen, USA). .. First-strand complementary DNA (cDNA) was subsequently generated from the purified RNA using a PrimeScript RT Master Mix kit (TaKaRa, Japan). qPCR amplification was carried out with the cDNA as the template on a QuantStudio 5 Real-Time PCR System (Applied Biosystems, USA) using TB Green Premix Ex Taq II (TaKaRa, Japan). ..

    Generated:

    Article Title: Deciphering IFN signaling in gastric cancer: A single-cell and bulk transcriptomic integration reveals CXCR4 as a key immunomodulator and prognostic determinant
    Article Snippet: Total RNA extraction was performed with Trizol reagent (Invitrogen, USA). .. First-strand complementary DNA (cDNA) was subsequently generated from the purified RNA using a PrimeScript RT Master Mix kit (TaKaRa, Japan). qPCR amplification was carried out with the cDNA as the template on a QuantStudio 5 Real-Time PCR System (Applied Biosystems, USA) using TB Green Premix Ex Taq II (TaKaRa, Japan). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Deciphering IFN signaling in gastric cancer: A single-cell and bulk transcriptomic integration reveals CXCR4 as a key immunomodulator and prognostic determinant
    Article Snippet: Total RNA extraction was performed with Trizol reagent (Invitrogen, USA). .. First-strand complementary DNA (cDNA) was subsequently generated from the purified RNA using a PrimeScript RT Master Mix kit (TaKaRa, Japan). qPCR amplification was carried out with the cDNA as the template on a QuantStudio 5 Real-Time PCR System (Applied Biosystems, USA) using TB Green Premix Ex Taq II (TaKaRa, Japan). ..

    Amplification:

    Article Title: Deciphering IFN signaling in gastric cancer: A single-cell and bulk transcriptomic integration reveals CXCR4 as a key immunomodulator and prognostic determinant
    Article Snippet: Total RNA extraction was performed with Trizol reagent (Invitrogen, USA). .. First-strand complementary DNA (cDNA) was subsequently generated from the purified RNA using a PrimeScript RT Master Mix kit (TaKaRa, Japan). qPCR amplification was carried out with the cDNA as the template on a QuantStudio 5 Real-Time PCR System (Applied Biosystems, USA) using TB Green Premix Ex Taq II (TaKaRa, Japan). ..

    Centrifugation:

    Article Title: Txnip regulates glycolysis and tricarboxylic acid cycle balance to maintain bone homeostasis.
    Article Snippet: .. Following sufficient grinding and centrifugation at 12,000×g for 10 min, the supernatant was combined with isopropanol for 5 min, and the subsequent procedures were conducted using the Kit. cDNA was synthesized from the RNA template using the PrimeScript RT Master Mix kit (Takara, Cat # RR036A) for reverse transcription. .. Gene expression was analyzed using the TBGreen® Premix Ex TaqTM kit (Takara, Cat # RR420A).

    Reverse Transcription:

    Article Title: Txnip regulates glycolysis and tricarboxylic acid cycle balance to maintain bone homeostasis.
    Article Snippet: .. Following sufficient grinding and centrifugation at 12,000×g for 10 min, the supernatant was combined with isopropanol for 5 min, and the subsequent procedures were conducted using the Kit. cDNA was synthesized from the RNA template using the PrimeScript RT Master Mix kit (Takara, Cat # RR036A) for reverse transcription. .. Gene expression was analyzed using the TBGreen® Premix Ex TaqTM kit (Takara, Cat # RR420A).



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